Read lengths very from short to over 100Kb.
We can run either instrument in CCS or CLR mode. An 8M SMRTcell can generate as much as 170Gbases of CLR DNA sequence data. Each 1M SMRTcell generates 15Gb of CLR DNA sequence data. Because the SMRT approach involves sequencing large, intact DNA segments, it dramatically improves or enables several genomics approaches, including: (1) de novo genome assembly, (2) detecting structural variants, (3) constructing full haplotypes, (4) more accurate transcriptome assembly, (5) detecting epigenetic modifications, and (6) direct RNA sequencing to measure post-transcriptional modifications and secondary RNA structure that is lost during cDNA synthesis. We are also running 2 Sequel instruments. In high output mode it generates some 2 billion reads per flow cell.
This machine can operate with twice this capacity in either Rapid Mode (2x2 lanes) or high output mode (2x8 lane). In Rapid Mode generates 125-150Gb of sequence data (2x250) per lane of a 2 lane flow cell, or runs a single flow cell (8 lane flow cell) in “High Output Run Mode” and generates 62.5 Gb of sequence data (2x125) per lane.
